Description
VWR Boekel 5420 Hybridization Oven 115V 320W Ambient+10°C to 100°C No Carousel
The VWR Boekel Scientific 5420 is a benchtop hybridization oven designed for rotating bottle hybridization and incubation assays. It features a motorized rotation drive with adjustable speed, a digital temperature controller, and a sealed insulated chamber for consistent temperature across the run. The unit holds up to ten 35 x 300mm hybridization bottles or up to twenty 35 x 150mm bottles.
Specifications & What's Included
- Manufacturer: Boekel Scientific / VWR Scientific Products
- Model: 5420
- Boekel Cat. No.: 230400
- VWR Cat. No.: 97005-252
- Voltage: 115V AC
- Current: 2.6A
- Power: 320W
- Frequency: 60 Hz
- Phase: Single Phase (1)
- Temperature Range: Ambient +10°C to 100°C
- Digital temperature display on front control panel
- Motor on/off switch and variable speed rotisserie knob on front panel
- Bottle Capacity: Up to 10 × 35 x 300mm bottles, or up to 20 × 35 x 150mm bottles
- Internal Chamber Dimensions: 13.25" x 9.75" x 9.5"
- Overall Dimensions: 17" x 17" x 16"
- Stainless steel interior chamber
- Glass-windowed insulated door
- IEC AC power inlet on rear panel
- Carousel: Not included
Applications
Hybridization ovens are standard equipment in molecular biology, genomics, and diagnostic laboratories for Southern, Northern, and Western blotting protocols, as well as in situ hybridization and membrane-based assays. The rotating bottle format keeps hybridization solution in continuous contact with the membrane or blot inside the bottle, which improves probe binding uniformity and reduces background. The Boekel 5420 is a well-regarded mid-capacity unit used in university research labs, clinical genetics labs, and pharmaceutical research settings. Its temperature range up to 100°C covers common hybridization conditions (typically 42°C–68°C for nucleic acid probes) as well as higher-temperature incubation applications. Units of this type remain in active use in labs maintaining Southern and Northern blotting workflows and in any setting requiring a compact, motorized incubation oven.
This VWR Boekel 5420 Hybridization Oven has been powered and tested at our facility and is in good working order. The heating function was tested and confirmed good up to 70°C, and the motor and its speed controls tested good as well — the unit powers on, heats up, and rotates normally. The carousel is not included with this unit. The door's rubber gasket is beginning to separate and will likely need to be reseated or replaced. The interior chamber appears clean, and the exterior shows normal wear consistent with lab use. Please view our photo set and contact us if you have any questions.
Warranty Information
Additional Information
Brand: |
Boekel Scientific / VWR |
Model: |
5420 |
MPN: |
230400 |
VWR Catalog Number: |
97005-252 |
Type: |
Hybridization Oven |
SKU: |
48522 |
Condition: |
Used – Inspected and Tested |
Seller Notes: |
No carousel included. Door gasket is loosening. |
Country/Region of Manufacture: |
United States |
Form Factor: |
Benchtop |
Power Requirements: |
115V AC, 2.6A, 320W, 60Hz, Single Phase |
Voltage: |
115V AC |
Wattage: |
320W |
Amperage: |
2.6A |
Frequency: |
60 Hz |
Phase: |
Single Phase (1) |
Temperature Range: |
Ambient +10°C to 100°C |
Application / Use Case: |
Hybridization, incubation, rotating bottle assays |
Bottle Capacity (35x300mm): |
Up to 10 bottles |
Bottle Capacity (35x150mm): |
Up to 20 bottles |
Internal Dimensions: |
13.25" x 9.75" x 9.5" |
Overall Dimensions: |
17" x 17" x 16" |
Carousel Included: |
No |
Compatible With: |
35x300mm and 35x150mm hybridization bottles |
Condition Notes: |
VWR Boekel 5420 Hybridization Oven tested good — heating confirmed to 70°C, motor and controls functional. Powers on, heats, and rotates well. No carousel included. Door rubber gasket is loosening and may need reseating. |